In addtion of increasing the boiling temp., DMSO weaken the douple strand
interactions. This should lower the annealing temp. and lower denaturing temp.
I would recomend playing with both parammeters if you want to use DMSO in PCR.
I have used DMSO before in PCR and was very effective. I found that
you need to find the optimum concentration for any DNA/primers combination.
DMSO inhibit taq activity at high concentrations.
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