reply

Juerg E. Frey juerg.frey at gw2.admin.ch
Fri Mar 24 11:22:53 EST 1995


Peter Thoren wrote:

>hi netters!

>I am going to test spermidine in order to improve my microsatellite PCR.
>I just want to know what final concentration is used by others.

>Thanks in advance,

>Peter Thoren
>Dept.of genetics, Uppsala University
>Sweden
>peter.thoren at genetik.uu.se   

The optimal range cited in a paper on this topic (Ching-Yi Wan and Thea A.
Wilkins (1993) Spermidine facilitates PCR amplification of target DNA. PCR
Methods and Applications 3: 208-210) is between 0.2 to 1.0 mM.

Regards - Juerg

Juerg E. Frey
Swiss Federal Research Station
for Fruit-Growing, Viticulture and Horticulture
CH-8820 Waedenswil
SWITZERLAND
PH: xxx411-783-6111
FAX:xxx411-780-6341
e-mail:  juerg.frey at wae.faw.admin.ch



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