There should not be a problem resuspending hemoglobin from ammonium
sulphate precipitate. Just macerate the precipitate with a glass rod and
water dropwise, while continuing to macerate. At some point, the
precipitate should dissolve into a clear solution.
You will now that the hemoglobin is not denatured if it is still red (the
binding of the heme group to the protein requires an intact molecule).
If I am not mistaken, TCA will denature hemoglobin irreversibly.
Falk Fish, Tel Aviv, Israel.
