now you see it now you don't

Kevin O'Donnell odonnell at sasa.gov.uk
Wed Nov 1 09:29:27 EST 1995


Rob Harling wrote:
> PCR works if we use freshly - prepared DNA, ie extracted from the 
> bacteria then run on the same day.  If we store the DNA at 4C for 
> only one week, then PCR fails, we get zilch in the lanes at any 
> concentration of template.  The DNA still looks the same after one week
> when run on a gel, ie there doesn't seem to be nuclease breakdown
> of the DNA.  Does anyone know what's happening to the DNA in storage?
> 
Rob,

Do you know if it still cuts with restriction endonucleases?

Kevin


Dr Kevin O'Donnell		     "Work as if you were in the early days
Diagnostics and Molecular Biology         of a better nation"  Alasdair Gray
Scottish Agricultural Science Agency    
Edinburgh                         		odonnell at sasa.gov.uk          
      




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