HSV with RT-PCR

Karl Fischer tyr-2 at bones.biochem.ualberta.ca
Fri Nov 3 18:11:34 EST 1995


In article <199511012009.NAA06359 at bock.ucs.ualberta.ca>,
Michele.Hales at UAlberta.CA (Michele Hales) wrote:

> When looking for HSV (A DNA virus) she has read published articles saying to
> run RT-PCR.  Once you get the PCR product are there any other
> protocols/procedures that need to be done?

Unless the source of the specimen contained cells which may harbour viral
RNA, it seems to me that the RT phase (and the specimen processing leading
to it) would be unecessary for detection of the virus by PCR.

Karl

-- 
Karl Fischer
tyr-2 at bones.biochem.ualberta.ca





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