precipitation problems

Dr Rob Harling ESA009 at ED.SAC.AC.UK
Mon Nov 6 11:40:14 EST 1995


> Date:          Sun, 05 Nov 95 01:47:35
> From:          <pohyam at technet.sg>
> Subject:       precipitation problems
> To:            mx%"diagnost at net.bio.net"
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> From: Lim Pohyam <pohyam at technet.sg>
> Subject: precipitation problems
> Date: 4 Nov 1995 08:30:10 -0800
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> My bacterial recombinant proteins precipitates upon long storage at -20C. 
> Upon thawing from freezer, I detected strands of precipitates. There is 
> difficulty to re-suspend these strands. As a result, when I set up my 
> microtiter plates, the activity is decreased. I sometimes encounter as much 
> as 15-20% drop in protein concentration. This is creating havoc in my 
> assays. I would like to receive feedback on how to remove this problem. what 
> can I do? I keep losing my protein everytime I thawed them from the freezer! 
> 
> 
> 
> 
Have you tried freeze-drying your proteins and redissolving from these?

Dr Rob Harling
SAC (Scottish Agricultural College)/
  University of Edinburgh
West Mains Road
Edinburgh EH9 3JG
Scotland, UK
tel: +44 (0)131 535 4000
fax: +44 (0)131 667 2601
e mail: esa009 at ed.sac.ac.uk




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