>Date: Fri, 15 Sep 1995 08:26:18 +0100
>From: "Alenka Kraigher, IVZ, +61 1323 245" <ALENKA.KRAIGHER at mf.uni-lj.si>
>Subject: Analysing Diphteria immunity
>Diphtheria outbreak in Eastern Europe shows that the risk of diphtheria is
>far from having disappeared.
>For this reason, in the National Institute for Public Health Slovenia we
>plan to check diphtheria immunity levels in the adult population before
>modification the diphtheria immunisation strategy to strengthen population
>immunity as a whole.
>>Among serological methods we chose indirect ELISA test (toxin-binding
>inhibition test, ToBI test) and in vitro neutralisation test on VERO cells.
>We found recommendation for those methods in WHO publications, but we could
>not find out where the products for executing these tests are produced.
>>May I ask if somebody could help us with information where preferably in
>Europe we could get products that are necessary for these tests.
>The products that we need for ToBI test are: diphtheria toxin, equine
>diphtheria antitoxin and peroxidase-labelled sheep antidiphtheria IgG.
>We got diphtheria reference serum from Statens Seruminstitut Copenhagen.
>Are there any kits for ToBI available? For in vitro neutralisation test we
>need: diphtheria toxin (purified) and horse hiperimmune diphtheria antitoxin,
>calibrated against WHO standard.
>>I will appreciate very much the information.
>>Dr. Alenka Kraigher
>PLEASE REPLY DIRECTLY TO: <ALENKA.KRAIGHER at mf.uni-lj.si>
--
Jack Woodall, Ph.D., ProMED List Moderator, New York State Dept.of Health,
Albany NY, USA
e-mail: jack.woodall at wadsworth.org
