Bluetongue Diagnostics

[William C. Wilson]

Several labs including mine have published procedures for detection of 
bluetongue and the related epizootic hemorrhagic disease viruses.  Attached 
is a partial listing of available references concerning BTV PCR.  There are 
also several published protocols for detection of African horse sickness 
that I didn't include for the sake of space.

Reference List

          1.        Akita, G. Y.; Chinsangaram, J.; Osburn, B. I.; 
Ianconescu, M., and Kaufman, R. Detection of bluetongue virus serogroup by 
polymerase chain reaction. J. Vet. Diagn. Invest. 1992; 4:400-405.
     2.        Akita, G. Y.; Glenn, J.; Castro, A. E., and Osburn, B. I. 
Detection of bluetongue virus in clinical samples by polymerase chain 
reaction. J. Vet. Diagn. Invest. 1993; 5:154-158.
     3.   Aradaib, I. E. Akita G. Y. Osburn B. I. Detection of epizootic 
hemorrhagic disease virus serotypes 1 and 2 in cell culture and clinical 
samples using polymerase chain reaction. J. Vet. Diagn. Invest. 1994; 
6:143-147.
     4.   Aradaib, I. E.; Wilson, W. C.; Cheney, I. W., and Osburn, B. I. 
Application of PCR for specific-identification of epizootic hemorrhagic 
disease virus serotype 2. J. Vet. Diagn. Invest. 1995; 7:388-392.
     5.   Aradaib, I. E.; Wilson, W. C.; Cheney, I. W.; Pearson, J. E., and 
Osburn, B. I. Application of PCR for specific identification of epizootic 
hemorrhagic disease virus serotype 2. Journal of Veterinary Diagnostic 
Investigation. 1995 Jul; 7(3):388-392.
     6.   Dangler, C. A.; de Mattos, C. A.; de Mattos, C. C., and Osburn, B. 
I. Identifying bluetongue virus ribonucleic acid sequences by the polymerase 
chain reaction. J. Virol. Meth. 1990; 28:281-292.
     7.   Katz, J. B.; Alstad, A. D.; Gustafson, G. A., and Moser, K. M. 
Sensitive identification of bluetongue virus serogroup by a colorimetric 
dual oligonucleotide sorbent assay of amplified viral nucleic acid. J. Clin. 
Microbiol. 1993; 31(11):3028-3030.
     8.   Katz, J. B.; Gustafson, G. A.; Alstad, A. D.; Adler, K. A., and 
Moser, K. M. Colorimetric Diagnosis of Prolonged Bluetongue Viremia in 
Sheep, Using an Enzyme-Linked Oligonucleotide Sorbent Assay of Amplified 
Viral Nucleic Acids. Amer. J. Vet. Res. 1993; 54(12):2021-2026.
     9.   ---. Colorimetric diagnosis of prolonged bluetongue viremia using 
an enzyme-linked oligonucleotide sorbent assay of amplified viral nucleic 
acids. J. Vet. Diag. Invest. 1993(In Press).
     10.  Maclachlan, N. J.; Nunamaker, R. A.; Katz, J. B.; Sawyer, M. M.; 
Akita, G. Y.; Osburn, B. I., and Tabachnick, W. J. Detection of bluetongue 
virus in the blood of inoculated calves: Comparison of virus isolation, PCR 
assay, and in vitro feeding of Culicoides variipennis. Arch. Virol. 1994; 
136(1-2):1-8.
     11.  McColl, KA and Gould, AR. Detection and characterisation of 
bluetongue virus using the polymerase chain reaction. Virus Res. 1991; 
21(1):19-34.
     12.  Mecham, J. O. Wilson W. C. Strategies for improved bluetongue 
diagnostic strategies for improved bluetongue diagnostics. Proc. U.S. Anim. 
Health Assoc. 1994; 98:43-50.
     13.  Wade-Evans A.M.; Mertens, PP, and Bostock, CJ. Development of the 
polymerase chain reaction for the detection of bluetongue virus in tissue 
samples. J. Virol. Methods. 1990; 30(1):15-24.
     14.  Wechsler, SJ; Austin, KJ, and Wilson, WC. Limits of detection of 
bluetongue virus with different assay systems. J. Vet. Diagn. Invest. 1990; 
2(2):103-6.
     15.  Wilson, W. C. Development of nested-PCR tests based on sequence 
analysis of epizootic hemorrhagic disease viruses non-structural protein 1 
(NS1). Virus Res. 1994; 31:357-365.
     16.  Wilson, W. C. and Chase, C. C. L. Nested and Multiplex Polymerase 
Chain Reactions for the Identification of Bluetongue Virus Infection in the 
Biting Midge, Culicoides-variipennis. J. Virol. Meth. 1993; 45(1):39-47.

Hope this is helpful.

  
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  William C. Wilson
  USDA-ARS
  Arthropod-borne Animal Diseases Research Lab.
  Laramie, WY  82071-3965

  Phone :     (307)766-3622
  Fax:            (307)766-3500
  E-Mail:      wcwilson at uwyo.edu

  
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