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storage of freeze dried fungal tissue

Gregory Forbes gaf2 at CORNELL.EDU
Tue Jan 9 15:33:23 EST 1996


The oomycete fungus, Phytophthora infestans, is an important plant pathogen
and is routinely studied with several biochemical and molecular markers.  A
convenient way of processing tissue for these analyses is to lyophilize it
and then grind it.  I would appreciate opinions as to whether this
lyophilized tissue would be best stored at room temperature or frozen.  It
seems to me that the apparent advantages of freezing the material could be
counter balanced by the increased risk of rehydration due to condensation in
the storage container (1.5 ml microfuge tubes), if the materials are thawed
and refrozen several times.

Thanks,

Greg
Greg Forbes,   plant pathologist,  International Potato Center (CIP)
Quito, Ecuador                                                   
 
Email:  gaf2 at cornell.edu
                                                                         
CURRENTLY:   Dept. Plant Pathology, Cornell University, 
334 Plant Science Bldg.   Ithaca, NY  14853
Fax:  +1(607)2554471   Tel:   +1(607)2553188




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