Many thanks to all the people who emailed me suggestions to my problems on
edge effects and storage of ELISA plates. I got over 30 responses! A common
theme was the idea of freezing plates after coating and blocking rather than
trying to dry the plate. I tried this and it has worked fine! My standard
curves are nearly identical on feshly prepared plates and on plates that have
been frozen after blocking.
I can say after quite a few attempts that there are some things to avoid;
drying plates, which in my hands decreases the max OD by about 1/4 and
increases interwell %cv's, and stacking plates which I suspect was what was
giving me my edge effects. I was stacking my plates 5-10 high.
Anyhow thanks again - it was great to see how a problem can be solved with
experts all around the world contributing.
Roger Collin
AgResearch
New Zealand
