In article <199601291418.OAA11091 at caird.scri.sari.ac.uk>,
djones at SCRI.SARI.AC.UK (D Jones) wrote:
>I'm presently doing some comparisons between different buffers to
dilute
>my labelled antibody in, for use in ELISA. So far I have established
so
>far is that the standard conjugate buffer that we use here (2% PVP,
0.2%
>Ovalbumin in PBS-T) can be improved upon with the addition of
>various other proteins such as BSA and Casein. I was wondering
whether
>anyone was using a buffer that they have found to be better than
>anything else they have tried, or does anyone know any commercial
>suppliers of conjugate/block buffers that they have found to be any
good.
>>Thanks
>David
>>
Can you give more information about what type
of conjugate you are trying to stabilize, and
what your current problems are. With more
info, I may be able to reach into my bag of
tricks and possibly help you out.
Best regards,
Les Confer
lconfer at moose.erie.net
