elisa

Steve Firth filter at firthcom.demon.co.uk
Wed Jun 12 16:45:17 EST 1996


In article <4pmouf$skv at news2.cts.com>,
b3748 at cts.com (Bryan Kiehl) wrote:

>In general you can divide these into serology or antigen detection.
>You can usually develop one protocol for serology. The key for
>serology is usually antigen quality. 

having once spent five years of my life devloping good tests for some of
these analytes, I'll "me too" Bryan's statement. To make a good test will
take a lot of time, and you must perform some painstaking titrations to get
good performance from your raw materials. I'd recommend going for a
commercial assay unless you have a lot of experience of immunoassay design
and standardisation.

Somehow though I have the feeling that if you had the experience required,
you probably wouldn't be asking for help.





More information about the Diagnost mailing list