I recently used the BLOTTO in my PCR reaction to amplify various genes from
soil DNA extracts. It works generally well in the presence of some
possible poly-phenolics compounds in brown colour.
Xiang
Dr. Xiang Li
West-East Centre for Microbial Diversity
University of British Columbia
BC, Canada
---------- Forwarded message ----------
Date: 25 Mar 1996 11:53:48 -0800
From: Solke Deboer <DEBOERS at em.agr.ca>
To: diagnost at net.bio.net
Subject: Milk in PCR mix
I understand some of you may be interested in our
experience with using BLOTTO (skim milk) in the
PCR mix to minimize inhibition due to the
presence of plant compounds. It was published in
Nuc. Acids. Res. 23 (1995), 2567-8. I'd be
interested to hear how well it works for you, or
doesn't.
Solke
