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Assaying MPO in granulocytes

Innovatek Medical Inc. Innovatek at mindlink.bc.ca
Thu May 2 02:58:15 EST 1996


We are developing an assay for myeloperoxidase (MPO) in human granulocytes.

Current concept is to isolate WBC from whole blood using Pall Leucocyte 
filter.  Then lyse the WBC to release the MPO and use hydrogen peroxide as 
substrate, TMB as chromogen.

But Pall leucocyte filter is very very expensive.  Anybody has any experience 
with other material for this purpose?

King F. Hui



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