Extraction of RNA from stool for RT-PCR

Dr. J.P. Clewley jclewley at hgmp.mrc.ac.uk
Tue Oct 1 04:25:48 EST 1996


Tong Kwok Leung <louistkl at netvigator.com> wrote:

>I am working on stools for RT-PCR of Norwalk viruses.
>I have tried four different methods of RNA extraction :
>1. Boom's method
>2. CTAB
>3. Promega's RNA extraction kit and
>4. Spin cartridge from Gibco.

>However, the results are not satisfactory. I don't know if there
>are any problems caused due to the extraction methods or because of
>the quantity of the related virus was too low.
>Can anyone give me advice on how to solve the above problems?

You could try phenol/chloroform extraction before Boom extraction;
make sure the stools are homogenised well before extraction.

Jon Clewley




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