Making Antibodies Let Go

Leigh Leigh at bangslabs.com
Tue Sep 3 03:24:42 EST 1996


Making Antibodies Let Go
If one is collecting cells by antibody binding, for instance, on a solid
surface, how does one recover the cells intact?

One idea is to use mechanical energy to rip the cells off the beads or solid
surfaces. It would seem that this might rupture significant numbers of cell membranes.
Other suggestions have been to let the cells multiply and daughter cells will
grow free of the solid support.

In the case of molecules I suppose it is even more important to recover the
molecules whole since it will be very difficult to rip these off the solid
surface without destroying them.  

Please share your tips for ways to make antibodies release antigens- whole molecules
or cells.

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Leigh Bangs, aka "The Particle Doctor(TM)";  leigh at bangslabs.com 
Bangs Laboratories, Inc., 979 Keystone Way, Carmel, IN 46032-2823 USA
Tel: 317-844-7176  Fax: 317-575-8801
"The Microsphere Zone"  [Web Site: http://www.bangslabs.com/blab]
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