Extraction of RNA from stool for RT-PCR

Tong Kwok Leung louistkl at netvigator.com
Mon Sep 30 04:19:44 EST 1996


I am working on stools for RT-PCR of Norwalk viruses. 
I have tried four different methods of RNA extraction :
1. Boom's method
2. CTAB 
3. Promega's RNA extraction kit and
4. Spin cartridge from Gibco.

However, the results are not satisfactory. I don't know if there
are any problems caused due to the extraction methods or because of 
the quantity of the related virus was too low.
Can anyone give me advice on how to solve the above problems?
Many thanks!



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