Shelf life of ELISA reagents

D.A.C. Jones djones at
Wed Apr 30 06:06:22 EST 1997

Does any body have any thoughts on the useful shelf life of ELISA 
reagents. I've recently been doing a series of experiments comparing the 
sensitivity of various ELISA protocols. One thing I have noticed, which 
is slightly annoying, is that on opening a new tin of skimmed milk 
(Marvel) and a new bottle of tween-20 (BDH) there are big reductions in 
the background. OK, so this may have been expected, but not to the extent 
that I have noticed. Both of the 'old' reagents had probably been opened 
less than 6 months ago. Any tips on storage etc?


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