Shelf life of ELISA reagents

Bryan Kiehl BKiehl at genbio.com
Thu May 1 03:23:04 EST 1997


I think you may want to consider more than stability. Contamination,
usually microbial type, of materials can participate in "background".
If not taken into account, you may also consider plastic changes,
assuming ELISA mean microtiter plates.
On 30 Apr 1997 04:06:22 -0700, "D.A.C. Jones" <djones at scri.sari.ac.uk>
wrote:

>Does any body have any thoughts on the useful shelf life of ELISA 
>reagents. I've recently been doing a series of experiments comparing the 
>sensitivity of various ELISA protocols. One thing I have noticed, which 
>is slightly annoying, is that on opening a new tin of skimmed milk 
>(Marvel) and a new bottle of tween-20 (BDH) there are big reductions in 
>the background. OK, so this may have been expected, but not to the extent 
>that I have noticed. Both of the 'old' reagents had probably been opened 
>less than 6 months ago. Any tips on storage etc?
>
>
>Regards
>Dave

Bryan Kiehl
GenBio, San Diego
BKiehl at GenBio.com



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