DNA isolation from fungal spores

yatika kohli kohliy at hotmail.com
Thu Sep 17 06:39:35 EST 1998


Dear Dr. O'Donnell,

You might find these two references to be helpful;
 1) Lee, S.B. and Taylor, J.W. 1990.  Isolation of DNA from fungal 
mycelia and single spores.  PCR protocols: A Guide to Methods and 
Applicaitons 282-287.

2) Kuske c.R. et al.,1998. Small-scale DNA sample preparation Method for 
field PCR detection of microbial cells and spores in soil.  Appl Environ 
Microbiol vol 64 (7): 2463-2472.
 
Hope this helps.

Cheers!

Yatika Kohli, Ph.D.
Project Scientist
Ontario Ministry of Health
Etobicoke, ON Canada.



>From biosci-request at net.bio.net Tue Sep 15 04:58:33 1998
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>From: "Kevin O'Donnell" <odonnell at sasa.gov.uk>
>Organization:  Scottish Agricultural Science Agency
>Date:          Tue, 15 Sep 1998 12:09:42 +0000
>Subject:       DNA isolation from fungal spores
>
>Can anyone suggest a good (i.e. rapid, simple) method for isolating 
>DNA from fungal spores? The quality of DNA has only  to be good enough 
for 
>PCR or LCR ampliciation, rather than cloning, orsequencing.
>
>Replies to the list please, rather than private email.
>
>Thanks,
>
>Kevin
>Dr Kevin O'Donnell                          
>Diagnostics and Molecular Biology    
>Scottish Agricultural Science Agency   Tel 0131 244 8924
>Edinburgh                                              Fax  0131 244 
8926
>EH12 8NJ   UK
>


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