Isoprostane ELISAs

Robert DiSilvestro disilvestroxxx.1 at
Tue Mar 30 04:05:21 EST 1999

Has anyone used the ELISA kits for isoprostanes?  I have some doubts about 
specificity, but a bigger concern is just performance.  The kit I have used 
from Assay Designs worked well at first, but has been very poor in the last 
year (ie bad spike and recovery, high and variable values for control 
subjects, and poor agreement between duplicates).  Anybody have any comments?

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