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Microbial DNA isolation from WBC with Chelex 100?

Mikhail Mikhail
Wed Oct 27 10:09:34 EST 1999


I am looking for a method of microbial DNA extraction from white cell
fraction of blood using the Chelex 100 resine.
I am particularly interested in isolation of C.pneumoniae DNA with
Chelex 100 for subsequent detection by PCR. However, I would appreciate
any reply from those who use Chelex for processing PCR blood samples.

Sincerely,

Michael Edelstein.
mailto: me at cliph.keytown.com





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