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bcl2 rearrangement

daemon at hgmp.mrc.ac.uk daemon at hgmp.mrc.ac.uk
Fri Feb 18 04:20:15 EST 2000


Hi 

we regularly assay for the t(14;18) rtanslocation which involves the
bcl2 oncogene and the IgH gene.  Our standard positive control is a PCR
product of approximately 230bp.  recently we obtained a patient sample
which gave a product of over 1000 bp.  
My question is: how big do they get?  is it possible that a false
negative could be obtained for very large and possibly non amplifiable
products.  Is there a diagnostic significance for very large rearranged
products?

thanks for your help

Louise
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