From tony.oconnor at may.ie Wed Feb 5 09:33:18 2003 From: tony.oconnor at may.ie (Tony O'Connor) Date: Mon Mar 7 06:31:12 2005 Subject: biotinylated protein for amplification Message-ID: I want to biotinylate a protein to use as an "amplification layer" in an ELISA, as follows: [Solid-phase]-(Ab)-(Ag)-(biotinylated Ab)-(streptavidin)- *{biotinylated protein}n*-(HRP-streptavidin)n I'm doing this to replace a biotinylated anti-streptavidin Ab which is not amplifying the signal. I'm going to try BSA - there's always BSA in the lab :-) Has anyone tried other proteins (adequately-sized with several non-adjacent accessible amino groups) in such a system? Thanks, Tony Tony O'Connor Institute of Immunology NUI Maynooth Ireland tony.oconnor@may.ie From ahopkins at neo.rr.com Wed Feb 5 09:33:51 2003 From: ahopkins at neo.rr.com (joe user) Date: Mon Mar 7 06:31:12 2005 Subject: test Message-ID: 0123456789 From hiv2003 at club-internet.fr Mon Feb 10 05:50:42 2003 From: hiv2003 at club-internet.fr (Int.Persistence.Workshop) Date: Mon Mar 7 06:31:12 2005 Subject: International HIV Persistence Workshop Message-ID: <20030207150823.70ECBE0EE@relay-4m.club-internet.fr> An HTML attachment was scrubbed... URL: http://iubio.bio.indiana.edu/bionet/mm/diagnost/attachments/20030210/57c933a5/attachment.html