May 2004

qPCR.Gene-Quantification.Info Newsletter gene.quantification at wzw.tum.de
Mon Jul 5 10:45:35 EST 2004



Gene Quantification Newsletter May 2004

A new URL is defined for the Gene Quantification home page

<http://www.gene-quantification.info/>http://www.gene-quantification.info



A lot of NEWS are presented:

On HKG and normalization page:
Guideline to reference gene selection for quantitative real-time PCR
Selection of appropriate control genes to assess expression of tumor 
antigens using real-time RT-PCR
Comparison of RiboGreen® and 18S rRNA quantitation for normalizing 
real-time RT-PCR expression analysis
Novel Internal Controls For Real-Time PCR Assays
Selection of optimal internal controls for gene expression profiling of 
liver disease

On real-time PCR page:
Real-time PCR in the microbiology laboratory
Quantitative RT-PCR: pitfalls and potential
Real-time RT-PCR: Neue Ansätze zur exakten mRNA Quantifizierung

On chemistry page:
<http://dyes.gene-quantification.info/>http://dyes.gene-quantification.info/

Short summary of detection formats
SYBR Green I
BEBO
TaqMan Probes
LightCycler Hybridisation Probes
Molecular Beacons
Scorpions
LNA Primers
Light-Up Probes
Q Zyme Assay
Amplifluor Quantitative PCR Detection System
LUX (Light Upon eXtension) primers
The iCycler iQ Detection System for multiplex real-time PCR Assays
A novel real-time quantitative PCR method using attached universal template 
probe



Determination of real-time PCR efficiency

All necessary informations about qPCR efficiency estimation
<http://efficiency.gene-quantification.info/>http://efficiency.gene-quantification.info


BioInformatics in real-time PCR

A lot of applications related to qPCR or real-time PCR
STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED
<http://bioinformatics.gene-quantification.info/>http://bioinformatics.gene-quantification.info



Endocrinology, Physiology and Immunology

Application of real-time PCR in molecular Physiology, molecular 
Endocrinology and molecular Immunology
<http://physiology.gene-quantification.info/>http://physiology.gene-quantification.info



The 1st international qPCR Symposium & Application Workshop

Please look at the nice pictures of the Symposium
<http://qpcr2004.gene-quantification.info/>http://qpcr2004.gene-quantification.info
and Workshop talks are FREE for download
<http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html>http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html


Please send the NEWSLETTER and the 
<http://www.gene-quantification.info/>http://www.gene-quantification.info 
home page links to further scientists and friends who are interested in 
real-time PCR, new quantification strategies, relative or absolute gene 
quantification using kinetic PCR.

best regards

Michael Pfaffl

#############################################

For further questions concerning real-time PCR and if you want to SUBSCRIBE 
to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact 
<mailto:gene.quantification at wzw.tum.de>gene.quantification at wzw.tum.de   You 
will receive all new features and future REST &  REST-XL  &  BestKeeper 
updates.

GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are 
educational sites with the only purpose of facilitating access to 
biology-related information over the internet. The web page is optimised 
for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and 
higher versions) with an optimal screen resolution of 1024 x 768 pixels.

Disclaimer & copyright on 
<http://www.gene-quantification.info/>http://www.gene-quantification.info 
homepage.

********************************************************************
www.Gene-Quantification.Info

mail:           gene.quantification at wzw.tum.de
homepage:       http://www.gene-quantification.info

PD Dr. Michael W. Pfaffl
Physiology - Weihenstephan
Life Science Center Weihenstephan,
Technical University of Munich
Weihenstephaner Berg 3
D-85350 Freising-Weihenstephan, GERMANY
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