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<html> <body> <h1>Gene Quantification Newsletter March 2004</h1>New URLs were defined for the Gene Quantification Main page <a href="http://www.gene-quantification.info/">http://www.gene-quantification.info</a> or <a href="http://qpcr.gene-quantification.info/">http://qpcr.gene-quantification.info</a> or <a href="http://www.real-time-pcr.info/">http://www.real-time-pcr.info</a> <h2>BioInformatics in real-time PCR</h2>and Determination of real-time PCR efficiency <a href="http://bioinformatics.gene-quantification.info/">http://bioinformatics.gene-quantification.info</a> <h2>Endocrinology, Physiology and Immunology</h2><a href="http://physiology.gene-quantification.info/">http://physiology.gene-quantification.info</a> <h2>The 1st qPCR Symposium & Application Workshop talks are FREE for download</h2><a href="http://qpcr2004.gene-quantification.info/">http://qpcr2004.gene-quantification.info</a> and <a href="http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html">http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html</a> New features on the Gene-Quantification web page: Determination of real-time PCR efficiency <a href="http://www.gene-quantification.info/efficiency.html">http://www.gene-quantification.info/efficiency.html</a> PCR Efficiency estimation via "experimental four parametric sigmoidal model fit" (including all fluorescence data points, Tichopad et al. MCP 2004 - Inhibition of real-time RTPCR quantification due to tissue-specific contaminants) <a href="http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-4">http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-4</a> PCR Efficiency estimation via "experimental four parametric logistic model fit" (including all fluorescence data points, Tichopad et al. 2003 - Standardized determination of real-time PCR efficiency from a single reaction set-up.) <a href="http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-43">http://www.gene-quantification.info/efficiency.html#e-fluoro-sig-3</a> KOD (kinetic outlier detection) in real-time PCR (Tzachi Bar et al., 2003) <a href="http://www.gene-quantification.info/efficiency.html#kod">http://www.gene-quantification.info/efficiency.html#kod</a> Effect of zinc deficiency on the mRNA expression pattern in liver and jejunum of adult rats: Monitoring gene expression using cDNA microarrays combined with real-time RT-PCR <a href="http://www.gene-quantification.info/zinc.html#array1">http://www.gene-quantification.info/zinc.html#array1</a> Identification of Genes Responsive to Intracellular Zinc Depletion in the Human Colon Adenocarcinoma Cell Line HT-29 <a href="http://www.gene-quantification.info/zinc.html#array2">http://www.gene-quantification.info/zinc.html#array2</a> The mRNA Expression of TWO Insulin Receptor Isoforms (IR-A and IR-B) and IGFR-2 in the Bovine Corpus Luteum During the Estrous Cycle, Pregnancy, and Induced Luteolysis <a href="http://www.gene-quantification.info/endo.html#insulinrec">http://www.gene-quantification.info/endo.html#insulinrec</a> Please send the NEWSLETTER and the home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR. best regards Michael Pfaffl For further QUESTIONS concerning real-time PCR and if you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact <a href="mailto:gene.quantification@wzw.tum.de">gene.quantification@wzw.tum.de</a>   You will receive all NEW FEATURES and future REST &  REST-XL updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are non-commercial, educational sites with the only purpose of facilitating access to biology-related information over the internet. The web page is optimised for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 pixels. <x-sigsep></x-sigsep> ******************************************************************************************** Gene-Quantification.Info mail:<x-tab>   </x-tab><x-tab>        </x-tab>gene.quantification@wzw.tum.de homepage:<x-tab>       </x-tab><a href="http://www.gene-quantification.info/" eudora="autourl">http://www.gene-quantification.info</a> PD Dr. Michael W. Pfaffl Physiology - Weihenstephan Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan GERMANY ******************************************************************************************** </body> </html>

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