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<html> <body> <h1>Gene Quantification Newsletter May 2004</h1>A new URL is defined for the Gene Quantification home page <a href="http://www.gene-quantification.info/">http://www.gene-quantification.info</a>   <h3>A lot of NEWS are presented:</h3>On HKG and normalization page: Guideline to reference gene selection for quantitative real-time PCR Selection of appropriate control genes to assess expression of tumor antigens using real-time RT-PCR Comparison of RiboGreen® and 18S rRNA quantitation for normalizing real-time RT-PCR expression analysis Novel Internal Controls For Real-Time PCR Assays Selection of optimal internal controls for gene expression profiling of liver disease On real-time PCR page: Real-time PCR in the microbiology laboratory Quantitative RT-PCR: pitfalls and potential Real-time RT-PCR: Neue Ansätze zur exakten mRNA Quantifizierung On chemistry page: <a href="http://dyes.gene-quantification.info/">http://dyes.gene-quantification.info/</a> Short summary of detection formats SYBR Green I BEBO TaqMan Probes LightCycler Hybridisation Probes Molecular Beacons Scorpions LNA Primers Light-Up Probes Q Zyme Assay Amplifluor Quantitative PCR Detection System LUX (Light Upon eXtension) primers The iCycler iQ Detection System for multiplex real-time PCR Assays A novel real-time quantitative PCR method using attached universal template probe <h3>Determination of real-time PCR efficiency</h3>All necessary informations about qPCR efficiency estimation <a href="http://efficiency.gene-quantification.info/">http://efficiency.gene-quantification.info</a> <h3>BioInformatics in real-time PCR</h3>A lot of applications related to qPCR or real-time PCR STATISTICS AND GENE EXPRESSION ANALYSIS by TERRY SPEED <a href="http://bioinformatics.gene-quantification.info/">http://bioinformatics.gene-quantification.info</a> <h2>Endocrinology, Physiology and Immunology</h2>Application of real-time PCR in molecular Physiology, molecular Endocrinology and molecular Immunology <a href="http://physiology.gene-quantification.info/">http://physiology.gene-quantification.info</a> <h2>The 1st international qPCR Symposium & Application Workshop </h2>Please look at the nice pictures of the Symposium <a href="http://qpcr2004.gene-quantification.info/">http://qpcr2004.gene-quantification.info</a> and Workshop talks are FREE for download <a href="http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html">http://qpcr2004.gene-quantification.info/qpcr2004-workshop.html</a>   Please send the NEWSLETTER and the <a href="http://www.gene-quantification.info/">http://www.gene-quantification.info</a> home page links to further scientists and friends who are interested in real-time PCR, new quantification strategies, relative or absolute gene quantification using kinetic PCR. best regards Michael Pfaffl ############################################# For further questions concerning real-time PCR and if you want to SUBSCRIBE to our monthly appearing GENE QUANTIFICATION NEWSLETTER please contact <a href="mailto:gene.quantification@wzw.tum.de">gene.quantification@wzw.tum.de</a>   You will receive all new features and future REST &  REST-XL  &  BestKeeper updates. GENE QUANTIFICATION web page and the GENE QUANTIFICATION NEWSLETTER are educational sites with the only purpose of facilitating access to biology-related information over the internet. The web page is optimised for Netscape Navigator version 4.7 & Internet Explorer version 5.0 (and higher versions) with an optimal screen resolution of 1024 x 768 pixels.   Disclaimer & copyright on <a href="http://www.gene-quantification.info/">http://www.gene-quantification.info</a> homepage. <x-sigsep></x-sigsep> ******************************************************************** <a href="http://www.gene-quantification.info/" eudora="autourl">www.Gene-Quantification.Info</a> mail:<x-tab>   </x-tab><x-tab>        </x-tab>gene.quantification@wzw.tum.de homepage:<x-tab>       </x-tab><a href="http://www.gene-quantification.info/" eudora="autourl">http://www.gene-quantification.info</a> PD Dr. Michael W. Pfaffl Physiology - Weihenstephan Life Science Center Weihenstephan, Technical University of Munich Weihenstephaner Berg 3 D-85350 Freising-Weihenstephan, GERMANY ******************************************************************** </body> </html>

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