reposting of "lacZ half life?"

mbyhsun%ccvax.dnet at LAS.AS.EDU.TW mbyhsun%ccvax.dnet at LAS.AS.EDU.TW
Sat Apr 24 12:44:22 EST 1993


From:	IN%"mbyhsun%ccvax.dnet at las.as.edu.tw" 18-APR-1993 10:21:07.22
To:	IN%"'dros at net.bio.net'%LAS.dnet at las.as.edu.tw"
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Subj:	lacZ half life

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Date: Sun, 18 Apr 93 10:17:28 -0800
From: mbyhsun%ccvax.dnet at las.as.edu.tw
Subject: lacZ half life
To: "dros at net.bio.net"%LAS.dnet at las.as.edu.tw
Message-id: <9304181817.AA14897 at las.as.edu.tw>

Does anyone have an estimate of the halflife of for lacZ mRNA and protein in
Drosophila? In particular, the P transposase-lacZ fusion by Bier et al (Genes &
Dev. 3:1273-1287, 1989)( a nuclear protein). What I am asking is basically what
is the temporal resolution when looking at the Xgal staining pattern to deduce
the lacZ transcription timing.
Thanks in advance for any information.

Y. Henry Sun
Institute of Molecular Biology
Academia Sinica
Taiwan
FAX (886-2)-782-6085



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