p element question

Kirsten Crossgrove kcrossgr at mail.sas.upenn.edu
Wed Oct 13 14:48:16 EST 1993


Hi,
I am about to try P-element mediated transformation for the first time.  I
am using a pCaSpeR construct with the hsp70 promoter and 3'trailer.  The
cDNA I cloned in has about 560 bp upstream of the ATG and it has 11 C's at
the 5'most end (artifact of the original cDNA cloning process).  Is there
any reason I should get rid of either the polyC region or the whole
upstream 560bp in terms of getting good expression of the transgene?  I'd
appreciate any advice. 
Thanks,
Kirsten Crossgrove
kcrossgr at mail.sas.upenn.edu



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