myc-tag staining

HAIYAN DENG hdeng at
Thu Jan 27 20:33:00 EST 1994

Hi everyone,
I am trying to do localization study for a protein of interest in Drosophila
embryo. The experiment is to generate a stable germ-line transformant to 
express in vivo the protein with myc-tag at the N-terminus. After the embryo 
is fixed, I stain the embryo with mAb 9E10 and subsequently with conjugated 
anti-mouse antibodies, followed by fluorescence microscopy. But I've been 
having difficulties with this experiment in that the fluorescence signal is 
extremely, although I can see fairly strong signal with western blot.

Any suggestion on how to improve the fluorescence localization experiment will
be greatly appreciated.
Many thanks.


More information about the Dros mailing list