Double-labelled in situs

Stephanie L. Fox slf8 at po.CWRU.Edu
Thu Jun 30 17:25:29 EST 1994

I am doing "in-situs" to whole embryos using digoxygenin-labelled
RNA probes and an antibody conjugated to alkaline phosphatase.
I would like to be able to detect the <engrailed> protein in
these embryos using an antibody to engrailed and a secondary
antibody conjugated to HRP.  I was wondering if anyone had a 
protocol for this.  I have seen this type of experiment in papers
but no one really said how they did it or even gave a reference.
I would appreciate your help.

Stephanie Fox
Case Western Reserve University
email: slf8 at

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