Looking for single-fly PCR DNA prep

Bill Engels wrengels at facstaff.wisc.edu
Wed Mar 23 01:27:54 EST 1994


In article <1994Mar21.161721.1 at eagle.wesleyan.edu>,
lappel at eagle.wesleyan.edu wrote:

> I would like to be able to do PCR from the genomic DNA of single adult
> Drosophila.  Has anyone got a protocol for a single fly DNA prep that 
> works reliably (stress those last two words) for PCR?


Have you tried the protocol in:

       Gloor, G. B. and W. R. Engels, 1992  Single fly DNA preps for 
       PCR. Dros. Inform. Ser. 71: 148-149.

The article is also in the first issue of DIN, available on FlyBase, and it
is described as well in (Gloor et al., 1993, Genetics 135:81-95). WeÕve
used this method on close to 10,000 individual flies now. The failure rate
is probably less than 1% for us.

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William R. Engels                     :     WREngels at facstaff.wisc.edu
Genetics Department, 445 Henry Mall   :         office: (608) 263-2213
University of Wisconsin               :            lab: (608) 262-5578
Madison, WI  53706                    :            FAX: (608) 262-2976
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