RAPDs in Drosophila

FLIES at myia.imbb.forth.gr FLIES at myia.imbb.forth.gr
Mon May 16 09:14:45 EST 1994

Here is some info on RAPDs in Drosophila. We isolated several RAPDs from mela-
nogaster to be used as "controls" for Anopheles gambiae RAPDs.  they 1)
seem to be as easy to score as in other organisms, 2) they can give you as
much trouble as in other organisms (reproducibility) and 3), based on reverse
genomic southerns, they look as if they contain unique sequences.  On the other
hand, from the sequence and in situ analysis of anopheles RAPDs we know that a
high percentage of the "unique" RAPDs contain,in addition to bona fide unique
sequences, a variety of simple repeats including oligo(GT)n. Several RAPDs were
linked to the An. gambiae microsatellite map (Biao and Kafatos) by recombi-
nation mapping (they behave as normal dominant markers).
As far as D. melanogaster is concerned, it does contain a lot (!) of simple
repeats of all kinds (incl. opa boxes, oligo(GT)n, oligo(GA)n, oligo(PuG)n,
oligo(GGATT)n etc.).  There are no reasons to beleive that these will be much
less polymorphic than in other organsims.
Finally, with reference to Tony Long's remarks on the availability of Droso-
phila sequences, the European genome mapping consortium has recently submitted
to the EMBL database more than 700 STS from D. melanogaster (about 140-150 Kb
of sequences). The entries include the respective cytogenetic location.

K. Louis
IMBB, Heraklion, Crete

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