DNA prep for P1 clones? Qiagen or PEG?
Charlie Wright Genetics
cw117 at mole.bio.cam.ac.uk
Tue May 17 05:57:26 EST 1994
I'm about to do 250ml (mini in the case of P1) preps of some of the P1
clones found on flybase. The only Protocol given says Grow O/N,
innoculate large flask, add IPTG to induce amplification, grow some more.
"proceed with the Qiagen maxi-prep according to the kit protocol". The
problem is, there isn't a Qiagen for P1 bacteriophage kit. I could treat
it like a really big plasmid, but does anyone know how the elution
buffers should be modified (if at all?) Help, help.
Others have suggested PEG precipitation.
Any experience appreciated.
Charlie
--
C. R. Wright Dept. of Genetics
+44 (0)223 333970 telephone Univ. of Cambridge
+44 (0)223 333992 telefax Downing Street, Cambs.
cw117 at mole.bio.cam.ac.uk CB2 3EH, England
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