deletion mapping by PCR?

Bill Engels wrengels at facstaff.wisc.edu
Sun Nov 20 00:17:18 EST 1994


In article <3ab9s7$pn4 at bigfoot.wustl.edu>, shaffer at biodec (Christopher
Shaffer) wrote:
>The
> idea is to use a suitable embryonic marker which would allow one to
> collect embryo's homozygous for the deletion chromosome.  These embryos
> could then be used in a PCR based assay looking for the presence of the
> gene.  If anyone has tried this technique I would appreciate any
> pointers. 


19 November 1994 

Yes, Carlos Flores in my lab did something like that. Except that it was
easier because we knew the deletion homozygotes lived to the pupal stage.
He used the Tubby marker to pick out pupae that had the deletions and
PCR¹d them with primers suspected of being within the deletion along with
some positive control primers. It worked fine.

\\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\ /X\
 \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \X/ \
William R. Engels                     :     WREngels at facstaff.wisc.edu
Genetics Department, 445 Henry Mall   :         office: (608) 263-2213
University of Wisconsin               :            lab: (608) 262-5578
Madison, WI  53706                    :            FAX: (608) 262-2976
----------------------------------------------------------------------



More information about the Dros mailing list