SL-2 Cell culture
riesenbe at medicine
Fri Sep 30 10:48:45 EST 1994
nwinegar at tuzo.erin (Neil Winegarden) writes:
Try calling Sigma Cell Culture tech. service at 800-848-7791.
Ask for Dr. Terry Johnson.
Regards, Brad Riesenberger riesenbe at medicine.wustl.edu
>I am having a problem with my SL-2 culture and was wondering if anyone
>has ever run into similar problems.
>I am growing cells in spinner flask cultures, using Shields and Sang's M3
>media and 10% FCS. Originally I had no problem getting the cells up to a
>good concentration and then all of a sudden, they stopped growing. They
>are not dead (I have done viability assays) yet they remain at a
>concentration which is only one third to half what I used to get them to.
>I have tried adding more serum, and glutamine to my media.
>I'm not sure what else to try.
>Any help would be greatly appreciated.
>University Of Toronto
>nwinegar at credit.erin.utoronto.ca
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