SL2 cells at 37

Neil Winegarden nwinegar at tuzo.erin
Wed Apr 5 18:02:16 EST 1995

First off, there is a perfect person to ask at NIH.  Talk 
to Dr. Carl Wu in the laboratory of Biochemistry NCI.  He studies Heat 
shock factor in Drosophila and could give some useful insight for your 

In addition, my experience would suggest that perhaps using 36 or even
35 C (for 15 to 20 minutes) would be more useful.  You will still get 
transcription from a heat shock promoter, and your cells will recover faster.

Hope that this is of some help,


Neil Winegarden
University Of Toronto
nwinegar at

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