Deficiency kit update

[Kathy Matthews]

The deficiency kits available through the Bloomington Stock 
Center change whenever new deficiencies appropriate for the kits 
become available to the collection.  We have recently updated our 
estimates of coverage using maps drawn in proportion to 
euchromatic DNA content.  We are grateful to John Merriam for 
generating the maps used for this reassessment of the deficiency 
kits.  Two sets of maps were used, one using the minimum 
breakpoint range for each deficiency, one using the maximum 
range.  Overall coverage is between 67% and 75% of the 
euchromatin.  Here are the results by chromosome or chromosome 
arm. 
 
           %            % Coverage    % Coverage 
      Euchromatin         Minimum       Maximum 
 
1        20.3                82            85 
2L       16.6                69            77 
2R       20.4                49            59 
3L       19.4                72            77 
3R       21.6                65            79 
4         1.5               100           100 
 
All      100                 67            75 
 
 
We are now in the process of using FlyBase to track down 
published deficiencies that will fill in some of the gaps in 
coverage.  When we finish contacting authors about these stocks I 
will post a list of the remaining gaps with the hope of 
identifying otherwise untraceable or unpublished deficiencies 
that might be available to the collection.   
 
A current list of deficiency kit stocks is always available from 
FlyBase in flybase/stocks/stock-centers/bloomington/df-kit.rpt2.  
Some additions and deletions were made to the kits in the process 
of reassessing coverage.  If you already have one or more kits, 
you can check for changes by comparing the stock numbers on the 
information sheet provided with the stocks to the df-kit.rpt2 
list on FlyBase.  From now on, in addition to updating the df- 
kit.rpt2 file on FlyBase, I will post a message to bionet.dros 
whenever stocks are added or removed from the deficiency kits.   
 
Deficiency kits can be ordered by chromosome or by arm.  You do 
not need to provide individual stock numbers; just ask for the 
deficiency kit for a given chromosome or arm.  Many of these 
stocks can be difficult to maintain so be prepared to provide 
close stockkeeping attention when requesting deficiency kits.   
 

--  Kathy

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Kathy Matthews			Bloomington Drosophila Stock Center
Department of Biology		MATTHEWK at FLY.BIO.INDIANA.EDU
Indiana University              MATTHEWK at INDIANA.EDU    
Bloomington, IN  47405-6801     812-855-5782