Myc tagging on polytenes
mjcann at med.cornell.edu
Fri Aug 25 17:14:16 EST 1995
In article <n1403034993.61221 at QuickMail.Yale.edu>,
Glenn.Doughty at QUICKMAIL.YALE.EDU ("Glenn Doughty") wrote:
> Myc tagging on polytenes
> A message from the BIG GUY 8/22/95 10:23 AM
> I have been unable to detect our Myc epitope in polytene chromosome
> preparations. (It works fine in both Westerns and tissue stainings.) Has
> anyone used Myc tagging in this way with any (or no) success? Are there any
> unique conditions which must be met? I am open to suggestions.
> Glenn Doughty
In the past, when I have used the myc tag, I have found it to be quite
sensitive to proteolytic cleavage. This may be a problem here so it may
be advantageous to switch to FLAG or SV5, or something similar.
Hope that helps.
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