Transfection and Schneider cells
gru at vms.huji.ac.il
Fri Dec 8 01:48:31 EST 1995
I am inquiring about transient transfection to Drosophila Schneider cells.
My questions are as follows:
1. The transfection methods: What are the suggested transfection methods to
transiently express genes in Schneider cells. Also, does anyone have
experience with lipophectin-mediated transient transfection in Schneider
2. The expression vectors: Our gene is cloned in mammalian expression
vector under the Alfa globin promoter and SV40 enhancer or under the
phosphglycerokinase promoter. Does anyone have experience with such
constructs in Drosophila cells ?
I will be also grateful for information regarding other vectors.
Thank you in advance,
Laboratory of Dr. Yosef Gruenbaum
Asheri at vms.huji.ac.il
Department of Genetics,
The Life Sciences Institute
Email: Asheri at vms.huji.ac.il
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