Embryo injections

Greg Gloor ggloor at julian.uwo.ca
Thu Dec 21 09:31:32 EST 1995


In article <4b983u$4pa at mserv1.dl.ac.uk>, <Per.Kylsten at mibi.su.se> wrote:

> Hello,
> I am interested to know if anyone out there is using delta 2-3 embryos as
> recipients for transgenic constructs, on a routine basis?
> If so, are there any important alterations to standard injection protocols
> to consider (other than the obvious omission of helper plasmid) ?
> 
> Thank you.
> 
> Per.Kylsten at mibi.su.se

We use genomic delta 2-3 all the time. Just make sure that you have a way
of eliminating the third chromosome and go for it. No other alterations
needed

-- 
Greg Gloor
Dep't Of Biochemistry, UWO



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