Transformation ?

Michael Forte forte at ohsu.edu
Thu Jul 6 12:28:31 EST 1995


In article
<Pine.A32.3.91.950705133257.126871A-100000 at lamar.ColoState.EDU>,
ashok at LAMAR.COLOSTATE.EDU (Ashok Mudgapalli) wrote:

> Dear Netters
> Does anybody had any success with Drosophila embryo transformation using 
> the plasmid purified on Qiagen Coloumns (Qiagen plasmid purification kit)?
> The plasmid prepared by this method looks better quality than CsCl 
> gradient purification, at least on EtBr gel.
> And also, does any body know the effect of EtBr contamination with the 
> plasmid (after CsCl purification) has any effect on the transformations? 
> 
> I greatly appreciate netters comments and suggestions.
> 
> Thanks in advance
> 
> Ashok Mudgapalli

Ashok,

We routinely use Qiagen purified DNA for transformation with, in my
opinion, better results than we used to get with CsCl purified DNA.

Mike Forte



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