Transformation ?

Ashok Mudgapalli ashok at LAMAR.COLOSTATE.EDU
Wed Jul 5 22:31:05 EST 1995


Dear Netters
Does anybody had any success with Drosophila embryo transformation using 
the plasmid purified on Qiagen Coloumns (Qiagen plasmid purification kit)?
The plasmid prepared by this method looks better quality than CsCl 
gradient purification, at least on EtBr gel.
And also, does any body know the effect of EtBr contamination with the 
plasmid (after CsCl purification) has any effect on the transformations? 

I greatly appreciate netters comments and suggestions.

Thanks in advance

Ashok Mudgapalli



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