orthophosphate labelling SL2 cells

[Frances Hannan Zoology]

rchun at helix.nih.gov (Rene Franz Chun) writes:

>I'm looking into labeling a phosphoprotein in our drosophila
>SL2 cells.  I found a Gibco serum free media w/ met and cys
>omited which should work for our 35S labelings.  But didn't
>see a phosphate free medium?  

I believe we get phosphate free medium specially made for us by Gibco 
here in the UK. I'm sure they could do it for you too.

-- 
Frances Hannan                                       
BILMS, Zoology, Downing St, Cambridge, CB2 3EJ, UK   
Phone (0223)336663, FAX (0223)461954                 
flh at mole.bio.cam.ac.uk