udolph at vzdmza.zdv.uni-mainz.de
Thu Jun 29 02:23:14 EST 1995
In article <9506280650.AA14752 at luxor.latrobe.edu.au>,
gendjc at LUXOR.LATROBE.EDU.AU wrote:
> (sorry if this is a repeat. We've been having a spot of bother)
> Hi all,
> I'm having fearful trouble removing the vitelline membrane from
> D. serrata (melanogaster group) embryos. Simulans is fine. I'm shaking in
> a 50/50 soln of n-heptane and 80% ethanol. If I use 100% methanol it
> makes no difference. I get very few falling out of the organic layer.
> Any suggestions?
> David Clancy
> la Trobe Uni.
> Melbourne, Australia
for D. melanogaster we use a combination of 50 % Heptane and 50% methanol
after fixation in an Eppendorf tube. First add the 500 µl heptane and than
the methanol. Shake for 30 seconds (we use a vortex). Devitelinized embryos
will sink down. Maybe this procedure works with your flies.
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