Drosophila stocks and DNA clones
matthewk at FLY.BIO.INDIANA.EDU
Fri Mar 10 09:21:37 EST 1995
I am posting this message for Burke Judd. -- KM
To: Friends of Flys
From: Burke Judd
Subject: Drosophila stocks and DNA clones.
Some of the following are unique combinations of mutants that will be
discarded when I leave the NIEHS, March 31, 1995. If you can use any
of them please let me know. Phone (919)541-4690; FAX (919) 541-7593;
e-mail judd_b at niehs.nih.gov.
z[v77h] w[+] is from Oregon-R
sc z w[zm]
y z[a] w[zm]
y z[a] w[zl]
sc z w[zvl]
In(1)w[m4], y sn
In(1)w[m4], spl sn
In(1)w[m4], y z
In(1)w[m4], z[v77h] sn
In(1)rst, y z
Df(1)Su(z)J93, y z / FM7 The distal breakpoint of this deficiency is
35 to 60 kb proximal to the w locus and extends through rst and vt but does
not include N. Deficiency acts as a dominant suppressor of z apparently
by acting on the w locus in cis. It also exhibits rst, vt, reduced viablity
and female sterility. From deletion mapping against various rst and vt
deficiencies, the suppressor of z element is proximal to rst-vt. Deficiency occurs at very low frequency as an ectopic exchange product from females
heterozygous for y w[sp-2] and z w[zm] or z w[zl]. Several strains
were recovered from both types of heterozygotes. Original recombinant
chromosomes contained the w[zm] or w[zl] alleles. These have been replaced
by crossingover with w[+] from Oregon-R or with w[65a25]. All versions of
these derivatives and the parental chromosomes are available. CaSpeR plasmid
clones of part of the region from +100 to +163 (white locus map).
transformed line carrying approx. 17.5kb extending from +122.5 to + 142
is available. Johng Lim, who did the transformation, also has a copy of
z[J91] This allele occurred spontaneously in z w[65a25] spl sn.
It causes lemon-yellow eye-color in z[J91] w[+] males and z[J91] w[+]/z[+] w[+] females. It acts only in cis, however, thus most likely is acting on the
z-w lethals: One allele of each of 13 lethals located in the region between
the z and w loci will be deposited in the Mid-America Stock Center at Bowling
Green. As many as three alleles for each locus will be kept here until the
end of March.
The deficiencies generated by ectopic recombination in the region flanking
the w locus that are described in Montgomery et al., (1991) Genetics 129:
1085-1098, will be available until the end of March.
echinus locus: We have cloned and sequenced genomic and cDNA that we believe
to be the ec locus. However, a transformed line carrying the genomic sequences fails to rescue ec mutations, thus we have not yet published this. Fly strains and clones are available to anyone who is interested in a collaboration to
complete this analysis. Contact Bibba Goode, Laboratory of Reproductive
Toxicology, NIEHS, P.O. Box 12233, RTP, NC 27709
More information about the Dros