ashok at LAMAR.COLOSTATE.EDU
Wed Nov 1 12:05:13 EST 1995
Have any body tried arranging the embryos on double stick tape at very
low density for microinjections? Probably my experiance may help some
folks who are doing microinjections.
After chemical (clorax) dechorination, I arranged the embryos at least
1mm apart on 13mm double stick tape. I did not exceed more than 12/cover
slip. When I did the injections (after dessication for appropriate time),
to my surprise I got more than 60% survival and from few slides I got
about 85% survival. I compared this with control which has about 25-30
embryos on 13mm tape. From controls I got about 10-15% survival. For
quite some time I was having trouble with good survival. I never got more
than 15% survival in the past. Now with this new method I am consistently
getting very good survival.
Probably some body can answer this mystery (at least mistery to
me).Why overcrowding the embryos on tape reduced the survival rate and
why there is a dramatic increase in survival after reducing the no. of
embryos per slide.
People who are currently involved in microinjections can try this
method and I appreciate sharing their experiances with netters.
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