re- myc tag

Kevin Edwards Kevin_Edwards at CELLBIO.DUKE.EDU
Thu Nov 2 12:24:05 EST 1995


                      Subject:                              Time:  12:18 am
  OFFICE MEMO         re: myc tag                           Date:  11/3/95

Tim from the Hartenstein lab wrote:
>I've heard that the myc epitope gives very weak/no embryonic staining.

In the Kiehart lab, we get good staining of myc-tagged nonmuscle myosin in
embryos. If the tag doesn't work for a given protein, it may be because the
tag becomes masked (or chewed off?). These problems would be very
construct-specific. 

Kevin Edwards  




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