Tell me how to prepare the 25kb plasmid DNA
Sat Nov 4 15:04:32 EST 1995
Hello,
My name is Dongkook Park, graduate student of Seoul National
Student and I'm studying the molecular genetics of Drosphila
melanogaster.
BTW, I have the obstacle to prepare the transposon plasmid for
transformation.
I have subcloned 15kb DNA fragment at the pCaSpeR vector.
So, I confirmed the clone through mini prep.
But, I does not prepare the more amount of it by medium prep.
Please, mail me how to prepare the large size DNA prep
whoever knows the tip.
My address is followed;
dkpark at power4.snu.ac.kr
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