Tell me how to prepare the 25kb plasmid DNA
Marc_Champagne at cellbio.duke.edu
Tue Nov 7 19:14:21 EST 1995
In article <47eluc$5o0 at sun330.snu.ac.kr>, () wrote:
> My name is Dongkook Park, graduate student of Seoul National
> Student and I'm studying the molecular genetics of Drosphila
> BTW, I have the obstacle to prepare the transposon plasmid for
> I have subcloned 15kb DNA fragment at the pCaSpeR vector.
> So, I confirmed the clone through mini prep.
> But, I does not prepare the more amount of it by medium prep.
> Please, mail me how to prepare the large size DNA prep
> whoever knows the tip.
> My address is followed;
> dkpark at power4.snu.ac.kr
Did you use the Promega wizard mini-preps? If so, I know that their silica
matrix sticks so much to the DNA that >20kb DNA won't get eluted. You might
want to check for a homemade prep (see Maniatis).
More information about the Dros